Gfp Stable Cell Line

Gfp was expressed under the constitutive cmv promoter.

Gfp stable cell line.

4t1 gfp cmv stable cells express transgenically gfp under the control of the cmv promoter. The green fluorescent protein gfp is a protein composed of 238 amino acid residues 26 9kda that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. As useful tools for scientific research reporter stable cell lines are those cells involved in expression of reporter protein s which are engineered through integrating the reporter expression cassettes into cell genomes mediated by plasmid or lentivirus vectors. Reporter stable cell lines.

4t1 gfp cmv stable cells t4 a mouse mammary gland tumor cell line has been widely used in cancer research and drug development in vitro and in vivo. Gfp reporter stable cell line hek293. Description transformed from sh sy5y cell line originated from a human bone marrow with neuroblastoma by lentivirus transduction this cell line express enhance egfp fluorescent marker. I am trying to transfect thp 1 cells with gfp lc3 to create a stably expressing cell line.

Selection markers can be delivered using the same plasmid that contains the gene of interest in cis or on a separate plasmid in trans that needs to be co transfected with the plasmid containing the gene of interest. However i can not get a very high transfection efficiency 10 and this expression disappears after 48hrs.